Figure 9.

Immunostaining of cell surface integrins on LN229 cells (A–C) and HT29 cells (D–F). Cells were stained with 50 nM of the indicated anti-integrin antibodies or isotype control antibodies in HBSS buffer containing 1 mM Ca²⁺ and 1 mM Mg²⁺ except for IPI-αVβ5.9 which used 1 mM Mn²⁺ and 0.2 mM Ca²⁺. After washing, integrin antibodies were detected using APC-conjugated goat anti-human secondary antibodies, Alexa Fluor 647 goat anti-rat IgG, or Alexa Fluor 647 goat anti-mouse F(ab’)2, and flow cytometry.