Table 2.
Antibody (Motif in CDR3) | Antigen | IgG cell surface immunostaining EC50 (nM) | IgG ectodomain SPR Kd (nM) | IgG competition of ectodomain binding to RGD mimetic Kd (nM) | IgG binding to ligand binding site on cell surface Kd (nM) | Fab binding to ligand binding site on cell surface KD (nM) | IgG inhibition of cell adhesion IC50 (nM) |
---|---|---|---|---|---|---|---|
IPI-αVβ3.7 (RGD) | αVβ3 | 3.7 ± 0.1 | 0.39 ± 0.08 | 0.76 ± 0.02 | 1.09 ± 0.46 | 12.0 ± 1.8 | 42.6 ± 9.1 |
αVβ8 | - | - | 2200 ± 800 | - | n.d. | - | |
IPI-αVβ3.13 (RLD) | αVβ3 | 2.3 ± 0.4 | 1.2 ± 0.1 | 2.6 ± 0.9 | 1.35 ± 0.36 | 38.8 ± 5.9 | 26.6 ± 4.9 |
IPI-αVβ5.9 (RGD) | αVβ5 | 0.76 ± 0.13 | 4.8 ± 0.4 | 2.4 ± 0.7 | 0.74 ± 0.28 | 41.9 ± 6.3 | 5.0 ± 0.1 |
αVβ3 | - | 490.5 ± 17.2 | 1700 ± 400 | 1200 ± 400 | n.d. | - | |
αVβ8 | - | - | - | 5200 ± 100 | n.d. | - | |
IPI-αVβ6.3 (RGD) | αVβ6 | 5.0 ± 2.0 | 2.3 ± 0.6 | 5.5 ± 0.5 | 0.99 ± 0.20 | 50.8 ± 4.8 | 2100 ± 500 |
αVβ8 | - | - | - | - | n.d. | - | |
IPI-αVβ6.4 (RTD) | αVβ6 | 5.7 ± 2.9 | 10.4 ± 4.7 | 2.2 ± 0.2 | 0.73 ± 0.14 | 60.9 ± 5.8 | 7400 ± 2800 |
αVβ8 | 7.2 ± 2.1 | 18.9 ± 7.1 | 11.3 ± 2.2 | 2.66 ± 0.41 | 131.2 ± 11.2 | 5.1 ± 1.1 | |
αVβ1 | - | - | 2400 ± 700 | - | n.d. | - | |
IPI-αVβ6.12 (RGD) | αVβ6 | 3.4 ± 1.6 | 2.2 ± 0.2 | 2.3 ± 0.3 | 1.08 ± 0.21 | 24.0 ± 2.3 | 1900 ± 400 |
αVβ8 | - | 386.9 ± 34.6 | 630 ± 140 | - | n.d. | - | |
αVβ1 | - | n.r.f. | 2800 ± 800 | - | n.d. | - | |
αVβ3 | - | - | 2300 ± 600 | - | n.d. | - | |
IPI-αVβ6.2 | αVβ6 | 3.4 ± 1.2 | 11.3 ± 0.5 | n.a. | n.a. | n.a. | 3700 ± 700 |
αVβ8 | - | 172 ± 36 | n.a. | n.a. | n.a. | ||
αVβ1 | - | n.r.f. | n.a. | n.a. | n.a. | - | |
IPI-αVβ8.1 | αVβ8 | 2.6 ± 0.6 | 0.27 ± 0.16 | n.a. | n.a. | n.a. | - |
IPI-αVβ8.8 | αVβ8 | 0.69 ± 0.20 | 1.6 ± 0.7 | n.a. | n.a. | n.a. | - |
IPI-α5β1.2 | α5β1 | 0.22 ± 0.05 | 1.9 ± 0.2 | n.a. | n.a. | n.a. | - |
IPI-α5β1.4 | α5β1 | 0.17 ± 0.06 | 4.3 ± 0.1 | n.a. | n.a. | n.a. | - |
αVβ6 | - | n.r.f. | n.a. | n.a. | n.a. | - |