TABLE 1.
Primers used in this study
| Designation | Sequencea |
|---|---|
| V heavy antisense | 5′-AGCGCTAGCTGAGGAGACGGTGAC-3′ |
| NheI | |
| V heavy sense | 5′-GAAACGCGTGTCCACTCCCAGGTCCAGCTGCAG-3′ |
| MluI | |
| V kappa antisense | 5′-ACTCGTACGTTTTATTTCCAACTTTGTCCCC-3′ |
| BsiWI | |
| V kappa sense | 5′-GACCACGATGTGATGTTGTGATGACCCAA-3′ |
| DraIII | |
| Hu IgG2,3 antisense | 5′-GGACGGATCCTCATTTACCCGGAGACAGGGAGAG-3′ |
| BamHI | |
| Hu IgG4 antisense | 5′-GGACGGATCCTCATTTACCCAGAGACAGGGAGAG-3′ |
| BamHI | |
| Hu IgG2,3,4 sense | 5′-CTGCTAGCCACCAAGGGCCCATCSGTCTTC-3′ S=C/G |
| NheI | |
| Hu IgA1 antisense | 5′-CGAGGATCCTCAGTAGCAGGTGCCGTCCAC-3′ |
| BamHI | |
| Hu IgA1 sense | 5′-CTAAGCTAGCCCGACCAGCCCCAAGGTC-3′ |
| NheI |
a
Restriction sites are in boldface type. Termination codons are in italics.