Figure 3.

Sickle cell mice exposed to RSD have higher density of DCX⁺GFAP⁺ astrocytes while having decreased densities of DCX⁺ neural progenitor cells and DCX⁺NeuN⁺ neurons in the hippocampus. A) DCX⁺ neural progenitor cell density. B) DCX⁺GFAP⁺ astrocyte cell density. C) DCX⁺NeuN⁺ neuron cell density D) Immunohistochemistry of DCX⁺ neural progenitor cells in AA mice. E) Immunohistochemistry of DCX⁺GFAP⁺ astrocytes in SS mice. F) Immunohistochemistry of DCX⁺NeuN⁺ neurons cells in AA mice. G) Immunohistochemistry of DCX⁺ neural progenitor cells in SS mice. H) Immunohistochemistry of DCX+GFAP+ astrocytes in SS mice. I) Immunohistochemistry of DCX⁺NeuN⁺ neurons in SS mice. Statistical comparisons were performed with a one-way ANOVA with Fisher’s LSD multiple comparisons test. *p<0.05, **p<0.01, ****p<0.0001. Data are presented as mean ± SD. ****p<0.0001.