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. 2024 Feb 1;134(3):e163145. doi: 10.1172/JCI163145

Figure 6. Ezh2 overexpression enhances optic nerve regeneration by transcriptionally suppressing Slc6a13.

Figure 6

(A) CUTTag followed by qPCR showing H3K27me3 enrichment in the promoter region of Slc6a13 (paired 2-tailed t test; P = 0.0161 between negative control and Slc6a13 R1, P = 0.0306 between negative control and Slc6a13 R2; n = 3 independent experiments). (B) Top: Experimental timeline. Bottom: Representative images of optic nerves showing that Slc6a13 overexpression partially blocks Ezh2 overexpression–induced optic nerve regeneration. Columns on the right display enlarged images of the areas in the white, dashed boxes on the left, showing axons at 250, 500, 750, and 1,000 μm distal to the crush sites, which are aligned with the yellow line. Yellow arrows indicate longest axons in each nerve. Scale bars: 100 μm, 50 μm for enlarged images. (C) Quantification of optic nerve regeneration in B (1-way ANOVA followed by Tukey’s multiple comparisons; P 0.0001 at 250, 500, 750, and 1,000 μm, P = 0.0010 at 1,250 μm; n = 8 mice for control, n = 10 mice for Slc6a13 overexpression, n = 7 mice for others). *P 0.05, **P 0.01, ***P 0.001, ****P 0.0001.