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. 2024 Feb 1;134(3):e163145. doi: 10.1172/JCI163145

Figure 8. Ezh2 overexpression enhances optic nerve regeneration by transcriptionally suppressing Lingo3 and Omg.

Figure 8

(A) Top: Experimental timeline. Bottom: Representative images of optic nerves showing that Lingo3 or Omg overexpression almost completely blocks Ezh2 overexpression-induced optic nerve regeneration. Columns on the right display enlarged images of the areas in white, dashed boxes on the left, showing axons at 250, 500, 750, and 1,000 μm distal to the crush sites, which are aligned with the yellow line. Yellow arrows indicate longest axons in each nerve. Scale bars: 100 μm, 50 μm for enlarged images. (B) Quantification of optic nerve regeneration in A (1-way ANOVA followed by Tukey’s multiple comparisons; P 0.0001 at 250, 500, 750, 1,000, and 1,250 μm, P = 0.0003 at 1,500 μm; n = 10 mice for control and Lingo3 overexpression, n = 9 mice for Ezh2 and Lingo3 cooverexpression, n = 7 mice for others). (C) CUTTag followed by qPCR showing H3K27me3 enrichment in the promoter region of Lingo3, but not that of Omg (paired 2-tailed t test; P = 0.0197 between negative control and Lingo3 R1, P = 0.1010 between negative control and Omg R1, P = 0.2329 between negative control and Omg R2; n = 3 independent experiments). Note that the negative control and positive control are identical to those in Figure 6A. *P 0.05, **P 0.01, ***P 0.001, ****P 0.0001.