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. 2024 Jan 31;626(7998):341–346. doi: 10.1038/s41586-023-06923-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a) Deamidation rate of COL1A1 508–519 for hominin specimens in the 2016–2022 and 1932–1938 excavations with ZooMS (layer X, n = 4 NISP; layer 8, n = 3; layer 9, n = 1), b) Glutamine (Q) deamidation plot for hominin specimens in the 2016–2022 and 1932–1938 excavations analysed with directDIA SPIN (layer X, n = 4; layer 8, n = 3), and c) Asparagine (N) deamidation plot for hominin specimens in the 2016–2022 and 1932–1938 excavations analysed with directDIA SPIN (layer X, n = 4; layer 8, n = 3). The hominin specimen recovered from layer 9 was not included in the SPIN analysis (plots b and c). For panel a, 1 indicates no deamidation of N or Q, while 0 indicates complete deamidation of N or Q. For panels b and c, 0 indicates no deamidation of N or Q, while 1 indicates complete deamidation of N or Q. The box plots within the violin plots define the range of the data (whiskers extend to 1.5× the interquartile range), outliers (black dots, beyond 1.5× the interquartile range), 25th and 75th percentiles (boxes), and medians (centre lines).