FIG. 4.

Effects of indomethacin and live bacteria on osteoclast numbers in mouse calvaria. Injections of indomethacin (40 μg in PBS) were given s.c. every 8 h for 6 days, beginning 2 h before the first bacterial injection and continuing until 4 h after the last injection in groups of mice (n = 5 for bacteria alone; n = 5, n = 6, and n = 7 for indomethacin with P. gingivalis, F. nucleatum, and C. rectus, respectively; n = 8 and n = 9 for RTF alone and RTF with indomethacin, respectively). Values are means ± standard errors of means. All values for the bacterium-injected mice (with and without indomethacin) were significantly different from values for the vehicle-treated (RTF) mice (P < 0.05). ∗, Significantly different from values for mice given bacteria alone (P < 0.01).