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. 2024 Jan 16;31(2):203–216. doi: 10.1038/s41418-024-01256-y

Fig. 3. ABLIM1 overexpression promotes CRC growth, migration, and invasion.

Fig. 3

A Western blot validation of the ABLIM1 overexpression efficiency in HCT116 and SW620 cells. B Cell proliferation indexes of HCT116 and SW620 cells transfected with pcDNA3.1-ABLIM1 or empty vectors were recorded by RTCA system. For HCT116, n = 4 for each group. For SW620, n = 3 for each group. C Cell migration indexes of HCT116 and SW620 cells detected by the RTCA system. For HCT116, n = 8 for each group. For SW620, n = 4 for each group. D For HCT116 and SW620 cells transfected with pcDNA3.1-ABLIM1 or empty vectors, the migrated cells in Transwell chambers were stained by 1% crystal violet solution. E Cell invasion indexes of HCT116 and SW620 cells transfected with pcDNA3.1-ABLIM1 or empty vectors detected by the RTCA system. Matrigel dilution ratios: HCT116, 1:10; SW620, 1:20. n = 8 for each group. F Cell invasion results of HCT116 and SW620 cells transfected with pcDNA3.1-ABLIM1 or empty vectors were visualized by crystal violet staining. For n > 3, paired student’s t-test; For n = 3, Friedman test. *P < 0.05; **P < 0.01; ***P < 0.001.