Skip to main content
. 2024 Jan 16;31(2):203–216. doi: 10.1038/s41418-024-01256-y

Fig. 4. ABLIM1 modulates IκBα/NF-κB/CCL20 activation.

Fig. 4

A The top 20 enriched KEGG pathways for differentially expressed genes (DEGs) between shABLIM1- and shVec-infected HCT116 cells. B The volcano plot displayed the up-regulated and down-regulated DEGs between shABLIM1- and shVec-infected HCT116 cells. The top 10 DEGs enriched with NF-κB signaling were labeled. C RT-QPCR results of ABLIM1 and the 10 DEGs. D Western blot images for ABLIM1, p65, IκBα, p-IκBα (ser36), CCL20, and GAPDH in ABLIM1- overexpressed or silenced HCT 116 cells. Relative IκBα/GAPDH protein levels were quantificated using 3 independent immunoblots. E Effect of ABLIM1 knock-down on cytoplasmic and nucleus p65 expressions in HCT116 and RKO cells. F Immunofluorescence images stained with anti-P65 antibody and DAPI showed ABLIM1 silence impaired p65 expression intensities in HCT116 and RKO cells. G Correlation analysis of ABLIM1 and RELA transcriptional levels in colorectal tumors and adjacent normal samples from the GEPIA2 database using Pearson method. P = 1.3e-8, R = 0.27. H Secreted CCL20 levels in culture supernatants of HCT116, RKO, and SW620 cells determined by ELISA kit. n = 3 for each group. I Representative p65, Ki-67, ABLIM1, and CCL20 immunohistochemical images of CRC xenografts from nude mice and the semiquantitative analysis upon H-score method. n = 5 for each group. For n > 3, paired student’s t-test; For n = 3, Kruskal–Wallis test. *P < 0.05; **P < 0.01; ***P < 0.001.