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. 2024 Jan 16;31(2):203–216. doi: 10.1038/s41418-024-01256-y

Fig. 5. IκBα/NF-κB p65 intervention reversed the phenotypes induced by ABLIM1 alteration.

Fig. 5

A Western blots for p65, p-P65, IκBα, p-IκBα, and ABLIM1 in HCT116 cells overexpressed with ABLIM1 or empty vector. Cells were treated with BAY11-7082 (10 μM) or DMSO control for 8 h before harvest. B Effect of BAY11-7082 treatment (10 μM, 24 h) on cell viabilities of HCT116 cells overexpressed with ABLIM1 or empty vector was detected by CCK-8 kit. n = 3 for each group. Kruskal–Wallis test. Influence of BAY11-7082 treatment on cell migration C and invasion D capabilities of HCT116 cells. E Western blots of P65 and ABLIM1 for validation of RELA overexpression efficiency in stable shABLIM1 or shVec HCT116 cells. F Cell viabilities assayed by CCK-8 kit revealed the effects of RELA overexpression on proliferation of stable shABLIM1 or shVec HCT116 cells. n = 3 for each group. Kruskal–Wallis test. Effect of RELA overexpression on HCT116 cell migration G and invasion H in Transwells. I RTCA monitored the influence of RELA overexpression on CRC cell proliferation, migration, and invasion abilities in HCT116 cells with or without ABLIM1 knockdown. n = 4 for each group. ***P < 0.001, indicated the statistical significances between shVec+Vec group and shABLIM1+Vec group at the end of assays. ###P < 0.01, indicated the statistical significances between shABLIM1+RELAOE group and shABLIM1+Vec group at the end of assays. Paired t test. J 48 h after transfection of IκBα S32/36 A phosphorylation mutant, full length ABLIM1, or both, NF-κB transcriptional activities in HCT116 and SW620 cells were detected by dual-luciferase assay. n = 3 for each group. Kruskal–Wallis test.