Fig. 8. HP domain deletion eliminates the oncogenic role of ABLIM1.

A Immunoblotting validated the overexpression efficiency of ABLIM1^WT, ABLIM1^∆HP, or empty vector in HCT116 cells. B HCT116 cell proliferation, migration, and invasion indexes monitored by RTCA system after overexpression with ABLIM1^WT, ABLIM1^∆HP, or empty vector. n = 4 ~ 6 in each group. Paired t-test. ^***p < 0.001 for ABLIM1^WT versus Vec. ^###p < 0.001 for ABLIM1^WT versus ABLIM1^∆HP. C HCT116 cell viabilities of indicated groups were assayed by CCK-8 kit after BAY11-7082 treatment. Kruskal–Wallis test: ^*P < 0.05; ^**P < 0^.01. D HCT116 cell migration and invasion images stained by crystal violet after indicated treatments. E A diagram showing how ABLIM1 mediates the activation of IκBα/NF-κB p65/CCL20 axis and thus augments CRC growth and metastasis.