FIG. 5.

(A) Western blot analysis of parasites recovered from a mouse chronically infected with Δgra2 parasites. Recovered parasites (Rec) lacked expression of GRA2, as did the original knockout clone that was injected (Inoc) and the wild-type line (WT). The membrane was probed with both MAb Tg17-179 against GRA2 (solid arrowhead) and a rabbit serum against T. gondii actin (asterisk) as an internal control of the quantity of protein in each lane. (B) Immunohistochemical staining of BAG5 in a cyst-like structure within the brain of a mouse chronically infected with Δgra2. Scale bar: 3 mm.