Fig. 1.
Antibody formats. (A) Complete IgG (immunoglobulin G) made up of VH and VL (the heavy and light variable domains of antibodies respectively) as well as the CL (constant domain of light chains) and CHn (constant domain of heavy chains) (B) Pepsin digestion cleaves IgG at a distinct site within the hinge region, resulting in a bivalent fragment that retains both binding sites, known as F (ab')2. (C) Papain digestion cleaves disulfide bonds located near the hinge region, producing a crystallizable fragment composed of the constant segments of two H chains (Fc) and two antigen-binding fragments (Fab). The schematic diagram was created with biorender.com.