Figure 3.

EPM uptake by 4T1 cells, evaluation of internalization mechanism, and EPM lethal impact on 4T1 cells

(A) Flow cytometry was used to quantify 4T1 cell EPM uptake at 0.5 h, 1.0 h, and 3 h.

(B) Laser confocal images of 4T1 cells uptake EPM at the above time points. Orange-red fluorescence represents Dir-labeled EPM, blue fluorescence (DAPI) represents cell nuclei, and the merge image shows the combination of orange-red and blue fluorescence. Scale bar, 100 μm. Data are represented as mean ± SEM. ∗ ∗ ∗p < 0.001.

(C) Flow cytometry was used to quantify 4T1 cell EPM uptake under different conditions, including 4°C, 37°C, chlorpromazine (100 μM), and amiloride hydrochloride (25 μM). Data are represented as mean ± SEM. ∗ ∗ ∗p < 0.001.

(D and E) Flow cytometry was used to detect the effect of EPM on 4T1 cell apoptosis. ∗∗p < 0.01, ∗∗∗p < 0.001.