Figure 4.

In vivo imaging and evaluation of therapeutic effect

(A) Representative time-dependent photoacoustic images in orthotopic breast cancer mice treated with EPM at predetermined time points during 24 h.

(B) The quantitative analysis intensities of tumor tissue (n = 3) determined by photoacoustic imaging at selected time intervals. Data are represented as mean ± SEM. ∗ ∗ ∗p < 0.001.

(C) Schematic depicting the experimental approach.

(D) Photographs of excised tumors (n = 5 per group) at day 26 after different treatments.

(E) Relative tumors growth curves (n = 5 per group). p values were obtained using ANOVA. ∗∗∗p < 0.001 by Student’s t test.

(F) Body weight curves (n = 5 per group).

(G–J) Detection of biomarkers of liver (ALT and AST) and kidney function (Scr and BUN) in mice (n = 3) with high-dose EPM injection.

(K) Representative pictures of H&E staining of major organ tissues in mice with high-dose EPM injection. Scale bar, 100 μm.