Table 2.

Single-cell CRISPR methods

Method		Reference
Perturb-seq	The Perturb-seq method combines a pooled CRISPR screen with single-cell RNA-seq by a guide barcode (GBC) expressed for each perturbation.	Prensner et al., 2021142
CRISP-seq:	The CRISP-seq technique allows the identification of the sgRNA that infects each individual cell by using a vector that contains the gRNA sequence and a transcribed polyadenylated unique guide index with a fluorescent selection marker.	Hanna and Doench, 2020141
Mosaic-seq: mosaic single-cell analysis by indexed CRISPR sequencing	Mosaic-seq couples CRISPRi with single-cell RNA sequencing. The library of vectors targeting enhancers also carries a unique barcode that allows the identification of the sgRNA.	Braun et al., 2016143
CROP-seq: CRISPR droplet sequencing	The CROP-seq method, unlike the other methods, does not pair the sgRNA with a barcode. Instead, the CROP-seq method uses the sgRNA as a barcode overlapping the Pol II transcript.	Manguso et al., 2017144

Summary of the different single-cell CRISPR methods currently available with a small description of the principle of the method.