Figure 3.

Traveling waves (TW) enhanced sarcomere maturation

(A) Representative confocal images of iCTs with or without TW on day 14. Cardiomyocytes were stained with anti-α-actinin, anti-TnT2, anti-Cx43, anti-β-MHC, and DAPI.

(B) Whole-cell lysates were extracted from TW and control groups and analyzed by western blotting using the indicated antibodies.

(C) TEM analysis of iCTs from both the TW and control groups on day 14. Z: Z lines; I: I band; T: T-tubules.

(D) The sarcomere length. Sarcomere lengths of CMs in both groups on day 14. (Mean ± SEM; Control: n = 4; TW: n = 3 biologically independent samples from two differentiations).