Figure 2.

Dmp1-expressing osteoblasts and osteocytes are increased in daβcat^Ot craniomaxillofacial (CMF) bones. Representative sagittal tissue sections of the maxillary edentulous ridge stained with DAPI from (A) control and (B) daβcat^Ot mice. Representative tissue sections stained with Goldner’s trichrome from (C) control and (D) daβcat^Ot mice. (E) Quantification of periosteal cell density, periosteal thickness, and osteocyte density. Coimmunostaining from (F) Dmp1 and (F′) β-catenin in controls. Coimmunostaining from (G) Dmp1 and (G′) β-catenin in daβcat^Ot mice. (H) Merged images of Dmp1 and β-catenin in daβcat^Ot CMF bone. Using Using Dmp1Cre^GFP/+ mice, immunostaining for GFP in (I) postnatal day 24 (P24) and (J) postnatal day 42 (P42) controls. GFP immunostaining in (K) P24 and (L) P42 daβcat^Ot mice. (M) Quantification of the percentage of GFP⁺ cells in a region of interest (ROI) from P24 and P42 controls (white and gray bars) and from P24 and P42 daβcat^Ot mutants (green bars). β-Catenin immunostaining on representative tissue sections from (N) P42 control and (O) daβcat^Ot mutant mice. (P) Quantification of β-catenin⁺ cells/total cells in a ROI and osteocyte shape, expressed as length and height of representative lacunae. Abbreviations as indicated in Fig. 1. ***P < 0.001.