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. 2023 Oct 24;14(2):362–379. doi: 10.1158/2159-8290.CD-23-0402

Figure 2.

Figure 2. Removal of mutant TP53 does not impact the proliferation, survival, mitochondrial content, and ROS levels in human cancer cell lines. A, In vitro growth of the indicated human cancer cell lines with or without doxycycline-mediated induction of a mutant TP53-specific inducible sgRNA (isgTP53) or an inducible control sgRNA (isgNC). B, In vitro survival of the cancer cells described in A. C, Cell-cycle analysis of the cancer cells described in A. D, Mitotracker staining of the cancer cells described in A. E, CellROX staining of the cancer cells described in A. The analyses described in C–E were conducted 2 days after the cancer cells had been treated with doxycycline for 5 days (see A). Data in A and B are presented as mean ± SEM of three independent experiments. There were no consistent significant differences between the mutant TP53–deleted cancer cells versus the control cancer cells in any of the experiments shown (see Supplementary Tables S1 and S2 for details of the statistical analyses).

Removal of mutant TP53 does not impact the proliferation, survival, mitochondrial content, and ROS levels in human cancer cell lines. A,In vitro growth of the indicated human cancer cell lines with or without doxycycline-mediated induction of a mutant TP53-specific inducible sgRNA (isgTP53) or an inducible control sgRNA (isgNC). B,In vitro survival of the cancer cells described in A. C, Cell-cycle analysis of the cancer cells described in A. D, Mitotracker staining of the cancer cells described in A. E, CellROX staining of the cancer cells described in A. The analyses described in C–E were conducted 2 days after the cancer cells had been treated with doxycycline for 5 days (see A). Data in A and B are presented as mean ± SEM of three independent experiments. There were no consistent significant differences between the mutant TP53–deleted cancer cells versus the control cancer cells in any of the experiments shown (see Supplementary Tables S1 and S2 for details of the statistical analyses).