Table 2.
The table shows the mean values of individual urinary peptide fragment abundance after placebo (n = 32) and dapagliflozin (n = 32) treatment periods, and the mean urinary levels of these peptide fragments in the independent cohort from healthy controls (n = 50) and DKD cases (n = 110). In addition, mass (in Daltons) and migration time (minutes) are given for each peptide fragment, as well as the log-2 transformed fold change induced by dapagliflozin and fold difference calculated between healthy controls and DKD cases in the validation cohort. The reported P-values were adjusted for multiple testing using the Benjamini-Hochberg method
| Human UniProt ID | Peptide sequence ID | Mass (Da) | CE-time (min) | Gene Symbol | Protein | Placebo (mean) | Dapagliflozin (mean) | Dapagliflozin ind. change | BH-adj. P-value | DKD (mean) | Healthy (mean) | DKD ind. change |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P04217 | 11163 | 2181.205 | 20.616 | A1BG | α-1B-glycoprotein | 257.834 | 19.502 | −3.725 | 0.019 | 46.823 | 0 | + |
| 12127 | 2423.331 | 21.122 | A1BG | α-1B-glycoprotein | 3617.333 | 758.044 | −2.254 | 0.027 | 2137.423 | 2.469 | 9.758 | |
| 12950 | 2310.242 | 20.925 | A1BG | α-1B-glycoprotein | 1196.521 | 306.267 | −1.966 | 0.031 | 1781.37 | 4.207 | 8.726 | |
| 13296 | 2493.302 | 19.608 | A1BG | α-1B-glycoprotein | 1052.219 | 33.214 | −4.984 | 0.031 | 379.197 | 0 | + | |
| P02765 | 8180 | 1792.936 | 21.267 | AHSG | α-2-HS-glycoprotein | 355.884 | 1001.749 | 1.493 | 0.042 | 213.442 | 0 | + |
| P02768 | 12518 | 1715.99 | 21.054 | ALB | Serum albumin | 54307.868 | 803.218 | −6.078 | 0.012 | 3134.85 | 28.44 | 6.784 |
| 12970 | 1829.053 | 21.335 | ALB | Serum albumin | 91459.779 | 959.985 | −6.573 | 0.012 | 2663.851 | 25.193 | 6.724 | |
| 13562 | 2540.269 | 19.687 | ALB | Serum albumin | 108201.088 | 236.708 | −8.828 | 0.012 | 7876.335 | 24.974 | 8.301 | |
| 14191 | 2752.422 | 19.881 | ALB | Serum albumin | 326527.011 | 1566.885 | −7.703 | 0.014 | 92739.29 | 423.45 | 7.775 | |
| 14790 | 1460.802 | 22.911 | ALB | Serum albumin | 14059.243 | 507.84 | −4.792 | 0.015 | 2015.093 | 7.35 | 8.099 | |
| 13726 | 2427.187 | 19.577 | ALB | Serum albumin | 10952.877 | 144.342 | −6.243 | 0.016 | 2015.244 | 21.418 | 6.556 | |
| 8491 | 2356.166 | 19.568 | ALB | Serum albumin | 3482.532 | 39.077 | −6.48 | 0.019 | 925.61 | 162.918 | 2.506 | |
| 8062 | 2639.32 | 19.823 | ALB | Serum albumin | 2556.829 | 19.993 | −7.002 | 0.027 | 6433.007 | 12.321 | 9.028 | |
| 7463 | 2566.365 | 19.53 | ALB | Serum albumin | 9486.713 | 19.01 | −8.966 | 0.038 | 2867.477 | 4.667 | 9.263 | |
| 5188 | 1777.952 | 21.01 | ALB | Serum albumin | 1971.827 | 52.188 | −5.238 | 0.045 | 35.267 | 0 | + | |
| P02656 | 19780 | 4113.847 | 24.548 | APOC3 | Apolipoprotein C-III | 178.772 | 476.177 | 1.413 | 0.016 | 354.975 | 506.002 | −0.515 |
| Q05682 | 7837 | 1753.934 | 21.086 | CALD1 | Caldesmon | 2558.923 | 58.12 | −5.461 | 0.035 | 1042.804 | 0 | + |
| P02452 | 17890 | 3416.589 | 31.958 | COL1A1 | Collagen α-1(I) chain | 590.236 | 1123.486 | 0.929 | 0.019 | 960.66 | 1241.408 | −0.377 |
| P08123 | 19745 | 4097.863 | 24.655 | COL1A2 | Collagen α-2(I) chain | 558.697 | 1274.198 | 1.189 | 0.02 | 1804.4 | 2939.349 | −0.713 |
| 13816 | 2825.275 | 24.449 | COL3A1 | Collagen α-1(III) chain | 9461.028 | 16079.857 | 0.765 | 0.011 | 15478.674 | 16458.099 | −0.089 | |
| 15237 | 2809.204 | 24.378 | COL3A1 | Collagen α-1(III) chain | 418.168 | 1003.36 | 1.263 | 0.012 | 608.266 | 679.408 | −0.152 | |
| P02461 | 15129 | 2583.168 | 23.633 | COL3A1 | Collagen α-1(III) chain | 90.217 | 210.593 | 1.223 | 0.02 | 347.915 | 286.865 | 0.275 |
| 8530 | 1834.836 | 24.214 | COL3A1 | Collagen α-1(III) chain | 490.291 | 824.621 | 0.75 | 0.027 | 1033.442 | 634.648 | 0.705 | |
| 11668 | 2248.99 | 26.156 | COL3A1 | Collagen α-1(III) chain | 10751.2 | 14269.938 | 0.408 | 0.031 | 13681.574 | 15627.938 | −0.184 | |
| Q9UBG3 | 883 | 974.524 | 20.641 | CRNN | Cornulin | 566.412 | 92.82 | −2.609 | 0.027 | 219.596 | 0 | + |
| Q8IY81 | 8534 | 1835.712 | 19.874 | FTSJ3 | pre-rRNA processing protein | 207.434 | 549.144 | 1.405 | 0.016 | 837.446 | 2049.9 | −1.286 |
| P08238 | 3285 | 1261.6 | 19.82 | HSP90AB1 | Heat shock protein HSP 90-β | 231.893 | 15.187 | −3.932 | 0.019 | 543.9 | 3.052 | 7.478 |
| B9A064 | 15015 | 2790.385 | 20.225 | IGLL5 | Immunoglobulin λ-like polypeptide 5 | 13096.651 | 106.472 | −6.948 | 0.037 | 22574.673 | 122.896 | 7.521 |
| P04264 | 9412 | 1934.792 | 19.905 | KRT1 | Keratin; type II cytoskeletal 1 | 185.944 | 384.677 | 1.049 | 0.027 | 564.75 | 1126.169 | −1 |
| Q6UXB8 | 8564 | 1838.932 | 20.941 | PI16 | Peptidase inhibitor 16 | 366.042 | 66.723 | −2.456 | 0.018 | 724.295 | 0 | + |
| P01833 | 18732 | 3669.665 | 24.075 | PIGR | Polymeric immunoglobulin receptor | 665.858 | 1579.226 | 1.246 | 0.014 | 1451.439 | 1045.853 | 0.475 |
| P41222 | 8589 | 1842.844 | 24.22 | PTGDS | Prostaglandin-H2 D-isomerase | 2263.559 | 148.626 | −3.928 | 0.045 | 586.969 | 700.124 | −0.252 |
| Q8WVN6 | 12387 | 2340.175 | 20.63 | SECTM1 | Secreted and transmembrane protein 1 | 436.162 | 55.053 | −2.986 | 0.037 | 234.333 | 17.235 | 3.766 |
| P01009 | 9486 | 1943.003 | 24.951 | SERPINA1 | α-1-antitrypsin | 9072.127 | 1854.2 | −2.291 | 0.031 | 9843.728 | 77.306 | 6.992 |
| 11408 | 2215.132 | 32.94 | SERPINA1 | α-1-antitrypsin | 4091.671 | 396.914 | −3.366 | 0.038 | 1826.854 | 0 | + | |
| Q9UPN9 | 10424 | 2067.824 | 20.597 | TRIM33 | E3 ubiquitin-protein ligase TRIM33 | 451.688 | 998.249 | 1.144 | 0.019 | 1329.369 | 2701.35 | −1.029 |
BH, Benjamini-Hochberg; CE-time, capillary electrophoresis migration time; DKD, diabetic kidney disease.