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. 2023 Nov 11;9(2):423–435. doi: 10.1016/j.ekir.2023.11.003

Figure 1.

Figure 1

IgA1 and Gd-IgA1 production by IgA1-producing cell lines derived from tonsils and the effect of LIF or OSM stimulation. (a) Experimental protocol for treatment of IgA1-secreting cells. Cells were stimulated with LIF (40 ng/ml), OSM (40 ng/ml), or control on day 0. Cell-culture medium was harvested on day 5 for determination of IgA and Gd-IgA1. (b) There were no differences in the concentrations of IgA in the cell-culture medium between cells from OSA versus patients with IgAN (Figure 1b, top). Treatment with LIF or OSM did not alter IgA concentration in the medium. For Gd-IgA1, baseline production was higher in the cells from patients with IgAN than from OSA group. LIF increased Gd-IgA1 production in the cells from patients with IgAN but not from OSA group. Treatment with OSM did not cause significant changes in OSA or IgAN group. One hundred Units of Gd-IgA1 was defined as 100 ng of the standard Gd-IgA1 (Figure 1b bottom). All data are presented as mean ± SD values and mean values for individual cell lines are shown by black circles. ∗P < 0.05; ∗∗P < 0.01. IgAN, IgA nephropathy; ns, not statistically significant; LIF, leukemia inhibitory factor; OSA, obstructive sleep apnea; OSM, oncostatin M.