Skip to main content
. 2023 Nov 11;9(2):423–435. doi: 10.1016/j.ekir.2023.11.003

Figure 3.

Figure 3

Impact of JAK2 inhibitor AZD1480 on Gd-IgA1 production and activation of STAT1 by LIF versus OSM in IgA1-secreting tonsillar cell lines. (a) LIF-induced overproduction of Gd-IgA1 in tonsillar IgA1-producing cells from patients with IgAN was inhibited by AZD1480 in a dose-dependent manner. (b) Conversely, OSM alone or OSM with AZD1480 did not alter Gd-IgA1 production. The cells were preincubated with the inhibitor for 60 minutes before addition of a cytokine. One-hundred Units of Gd-IgA1 was defined as 100 ng of the standard Gd-IgA1. (c) LIF enhanced phosphorylation of STAT1 (pSTAT1) and this effect was downregulated by AZD1480 in a dose-dependent manner. (d) Conversely, OSM or OSM with AZD1480 did not alter pSTAT1. In panels c and d, the cells were incubated with LIF or OSM for 30 minutes. Actin blots are shown as additional controls for protein load. (e and f) Densitometric analysis of pSTAT1 of data from b and d was performed for each sample (n = 3). pSTAT1 relative to total STAT1 level in OSA control cells (control) was set to 1. Statistical analysis for (a, b, e, and f) was performed by 1-way analysis of variance followed by Tukey’s multiple comparison test. All data are presented as mean ± SD values and mean values for individual cell lines are shown by black circles. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.005. IgAN, IgA nephropathy; ns, not statistically significant; LIF, leukemia inhibitory factor; OSA, obstructive sleep apnea; OSM, oncostatin M.