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. 2023 Nov 11;9(2):423–435. doi: 10.1016/j.ekir.2023.11.003

Figure 5.

Figure 5

STAT1 siRNA knock-down reduced LIF-induced Gd-IgA1 overproduction. (a–c) Quantitative polymerase chain reaction analysis of STAT1 gene expression (a) and SDS-PAGE/Western blotting of STAT1 protein (b and c) showed that siRNA knock-down of STAT1 decreased the expression of STAT1 in the cells from OSA and patients with IgAN. Actin blot is shown as an additional control for protein load. (d) siRNA knock-down of STAT1 reduced LIF-mediated overproduction of Gd-IgA1 in IgAN-derived cells. siRNA knock-down of STAT1 did not affect production of Gd-IgA1 after being stimulated with LIF in OSA-derived cells. One hundred Units of Gd-IgA1 was defined as 100 ng of the standard Gd-IgA1. Statistical analysis for (d) was performed by 1-way analysis of variance followed by Tukey’s multiple comparison test. All data are presented as mean ± SD values and mean values for individual cell lines are shown by black circles. ∗P < 0.05; ∗∗P < 0.01. IgAN, IgA nephropathy; k/d, knock-down; ns, not statistically significant; OSA, obstructive sleep apnea.