Greenness evaluation of the proposed method according to the AESa.
| Category | PPs (tap water) | Category | PPs (blood serum) |
|---|---|---|---|
| Reagents | |||
| Water | 0 | Water | 0 |
| Ferric nitrate | 6 | Ferric nitrate | 6 |
| Chloroform | 6 | Chloroform | 6 |
| Ethanol | 4 | ||
| Instruments | |||
| Spectrofluorometer | 0 | Spectrofluorometer | 0 |
| Occupational hazard | 0 | Occupational hazard | 0 |
| Waste | 6 | Waste | 6 |
| Total PPs: 18 score: 82 | Total PPs: 22 score: 78 | ||
a
PPs: penalty points. The assessment was conducted on a per-sample basis. Scores exceeding 75 are classified as excellent green analysis, scores within the range of 50–75 are considered acceptable green analysis, and scores below 50 are deemed inadequate green analysis.