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Visualization of LPSs of GR-12, mutants, and complemented mutants. (A) SDS-PAGE. Lanes: 1, GR-12 (wild type); 2, SMB213 (kfiC mutant); 3, SMB122 (rol mutant); 4, SMB122(pRAB10); 5, SMB43 (rfbKM mutant); 6, SMB43(pSMB109); 7, SMB20 (rfbD mutant); 8, SMB20(pGH1610); 9, SMB316 (kfiC-rfbD double mutant). (B) Tricine-SDS buffer system. Lanes: 10 and 13, GR-12; 11, SMB20 (rfbD mutant); 12, SMB43 (rfbKM mutant).
