TABLE 1.
Strains used in this study
| Strain | Descriptiona | Reference or source |
|---|---|---|
| GR-12 | Wild-type O75+ K5+ strain | 57 |
| XL1-Blue | F′::Tn10 proA+B+ lacIq Δ(lacZ)M15/recA1 endA1 gyrA96 (Nalr) thi hsdR17 (rK− mK+) supE44 relA1 lac | Stratagene |
| SY327 (λ pir) | F−araD Δ(lac pro) argE(Am) recA56 rpoB gyrA λ pir | 38 |
| SM10 (λ pir) | thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu Kmr λ pir | 53 |
| DH1 | F−supE44 recA1 endA1 gyrA96 (Nalr) thi-1 hsdR17 (rK− mK+) relA1 spoT1? rfbD1? | 31 |
| SMB20 | GR-12 rfbD::cat Cmr O75− K5+ | This study |
| SMB43 | GR-12 rfbK′-′M::cat Cmr O75P K5+ | This study |
| SMB122 | GR-12 Δrol::cat Cmr O75R K5+ | This study |
| SMB213 | GR-12 kfiC′::cat Cmr O75+ K5− | This study |
| SMB316 | GR-12 kfiC′::aphA rfbD::cat Cmr Kmr O75− K5− | This study |
a
Superscript P, partial O-antigen subunit; superscript R, random distribution of O-antigen chain lengths.