TABLE 3.

Oligonucleotide primers used in this study

Gene ampli- fied	Product size (kb)	Primer set	Sequencea
rfbD	2.5	rfbD F	5′-GTGGAATGGTATCTGTC-3′
		rfbD R	5′-TTGTGTTATAGATGTGC-3′
rol	0.7	rol F	5′-TGGCAAGATGACAATT-3′
		rol R	5′-AAGTTTATCTCAACGTCAAG-3′
ugd	1.1	ugd F	5′-CCGTCACGCGTTGCTATGCTG-3′
		ugd R	5′-TTGGATCCCCGTGAATGCTATAGG-3′b
his	0.8	his F	5′-TAGGATCCCCGAAGAAAGCAATTCCTTG-3′b
		his R	5′-TATCTAGACGGGCAATTTCCTCAATG-3′c
kfiC	3.2	kfiC F	5′-GGGAAGCTCTATTCTCAGTCGACG-3′d
		kfiD R	5′-CGCGACTATAGACTTTGTCGACC-3′d

^aSequences complementary to gene amplified are in boldface. 

^bBamHI sites are underlined. 

^cXbaI sites are underlined. 

^dSalI sites are underlined.