Figure 4.

Metagenomic diversity in control and cold stressed chickens at 21 d. Chickens were cold stressed at 4, 5, and 6 d of age as described in Methods. (A) Principal coordinate analysis plot based on Aitchison distances exhibits significant separation between gene family abundances of control and cold stress chickens at 21 d. (B) Differentially abundant gene families between control and cold stress chickens at 21 d. Effect size is defined as the between group differences divided by the within group differences, an effect size cut-off of absolute 1 is suggested for reproducible results. (C) Functional enrichment analysis of pathways based on differentially abundant gene families.