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. 2024 Jan 10;103(3):103393. doi: 10.1016/j.psj.2023.103393

Figure 6.

Figure 6

Serotonergic gene expression is altered in the chicken ceca following cold stress. Chickens were cold stressed at 4, 5, and 6 d of age as described in Methods. mRNA fold change of (A) 5-Hydroxytryptamine Receptor 7 (HTR7), (B) 5-Hydroxytryptamine Receptor 2C (HTR2C), and (C) Sodium dependent serotonin transporter (SLC6A4) were determined in full thickness chicken ceca tissue (n = 8-10 chickens per group) using RT-qPCR as described in Methods. Outliers were assessed using ROUT (q = 1%), and data analyzed using 2-way ANOVA with outliers removed followed by Sidak post-hoc test. Data are presented as mean ± SEM. Statistical significance was set at P < 0.05; * = P < 0.05; *** = P < 0.001.