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. 2024 Feb 8;30(2):e14574. doi: 10.1111/cns.14574

FIGURE 3.

FIGURE 3

Flutamide reduces PBMT‐mediated upregulation of TJ proteins in PT‐stroke rats and OGD‐treated bEND.3 cells. (A) Representative fluorescence images of ZO‐1 (green), Claudin 5 (green), and Reca1 (red) in the peri‐infarct areas. (B) Representative immunofluorescence images for Occludin (red), Claudin 5 (green), and Reca1 (red) in bEND.3 cells. Nuclei were counterstained with DAPI (blue). The data are expressed as mean ± SD (n = 5–6). (C, D) Quantitative analysis of vessel‐associated ZO‐1 and Claudin 5 were performed using ImageJ software. (E–G) Western blotting and quantitative analysis of the ZO‐1 and Claudin 5 levels using protein samples from the peri‐infarct brain region. The data are expressed as mean ± SD (n = 3). (H, I) Quantitative analysis of Claudin 5 and Occludin immuno‐intensity in bEND.3 cells. The data are expressed as mean ± SD (n = 5–6). (J) Western blotting and quantitative analysis of the Claudin 5 proteins in bEND.3 cells. The data are expressed as mean ± SD (n = 3). One‐way ANOVA followed by post hoc Bonferroni's test was used for analysis. * indicates p < 0.05 vs. Sham group or Control group; # indicates p < 0.05 vs. PT‐stroke group or the OGD group; & indicates p < 0.05 vs. PT + PBM group or OGD + PBM group. “n.s.” indicates no significant difference (p > 0.05). OGD, Oxygen‐glucose deprivation. Scale bar for tissue = 20 μm. Scale bar for cells = 10 μm.