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Relationship between growth temperature and expression of the ChoP epitope. P. aeruginosa PAO1 was grown to stationary phase at the temperature indicated, and equivalent numbers of cells in whole-cell lysates were examined by Western analysis on SDS-PAGE with MAb TEPC-15, which recognizes ChoP. Where indicated, following growth to stationary phase, the growth temperature was shifted for 4 h prior to the preparation of whole-cell lysates. Molecular sizes are in kilodaltons.
