Table 2. Thermal Melting Temperature Change of PA_N Endonuclease Variants with BXA and 23 (ΔT_M, °C)^a.

	DMSO	BXA (200 μM)	23 (200 μM)	23 (1 mM)
	TM (°C)	ΔTM (°C)	ΔTM (°C)	ΔTM (°C)
WT	58.7 ± 0.11	22.9 ± 0.08	17.8 ± 0.10	19.6 ± 0.16
I38T	59.6 ± 0.08	14.9 ± 0.04	12.9 ± 0.09	15.6 ± 0.28
E23K	61.5 ± 0.08	19.8 ± 0.06	10.2 ± 0.08	14.3 ± 0.09
A36V	51.3 ± 0.20	21.7 ± 0.11	16.4 ± 0.14	17.1 ± 0.12

^aExperiments with BXA and 23 were averaged from eight independent measurements.