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. 2024 Feb 7;16:37. doi: 10.1186/s13098-023-01210-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characterization of Exos collected from BMSC recollection medium. (A) BMSCs displayed a classical cobblestone-like morphology. (B-G) Immunofluorescence staining for cell surface markers. Phycoerythrin-conjugated antibodies are shown in red (PE, red). CD90, CD105, CD44 and CD29 are positive, whereas vWF is negative. (H-I) The differentiation potential of BMSCs was assessed by alkaline phosphatase (H) and oil red O staining (I). (J) Transmission electron micrographs showing BMSC-Exo morphology. (K) The particle size distribution in purified BMSC-Exos was determined by NanoSight. (L) Representative western blots showing CD43 and CD81 expression levels in BMSC-Exos.