TABLE 1.
Plasmids and bacterial strains
| Plasmid or strain | Description |
|---|---|
| Plasmids | |
| pDL289 | E. coli-streptococcal shuttle vector (7) |
| pMAJJ8 | pDL289-derived plasmid containing internally deleted spaP encoding amino acids 1 to 825 and 997 to 1561 (31) of P1 |
| pMAD | pDL289-derived plasmid containing PCR-amplified spaP encoding full-length P1 |
| pMal-p | Vector for expression of MBP fusions (NEB) |
| pMA3 | pMal-p-derived plasmid containing PCR-amplified DNA encoding amino acids 819 to 1017 (31) of P1 |
| pQE30 | Vector for expression of histidine-tagged polypeptides (QIAGEN) |
| pCG2 | pQE30-derived plasmid containing internally deleted spaP encoding amino acids 39 to 825 and 997 to 1561 (31) of P1 |
| pCG14 | pQE30-derived plasmid containing PCR-amplified spaP encoding amino acids 39 to 1561 (31) of P1 |
| Strains | |
| M15(pREP4) | Host strain of E. coli for pQE30-derived plasmids (QIAGEN) |
| CG2 | M15(pREP4) harboring pCG2 |
| CG14 | M15(pREP4) harboring pCG14 |
| PC3370 | spaP-negative mutant derived from S. mutans NG8 (12) |
| PC3370A | PC3370 transformed with pDL289 |
| PC3370B | PC3370 transformed with pMAJJ8 |
| PC3370C | PC3370 transformed with pMAD |