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Characterization of the LOSs expressed by wild-type, mutant, and complemented mutant strains of H. ducreyi. LOS present in proteinase K-treated whole-cell lysates was resolved by SDS-PAGE and either stained with silver (panel 1) or transferred to nitrocellulose for Western blot analysis with the H. ducreyi LOS-specific MAb 3E6 (panel 2). Lanes: A, wild-type strain 35000; B, rfaK (lbgB) mutant 35000.7; C, isogenic gmhA mutant 35000.252; D, 35000.252(pLS88); E, 35000.252(pLS253).
