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. 2024 Jan 24;17:1329738. doi: 10.3389/fnins.2023.1329738

TABLE 2.

fNIRS research on patients.

References (Region) Year Characteristics of participants Type of intervention fNIRS Parameters fNIRS paradigm Brain region Main findings
Experimental group Control group Experimental group Control group
Cognitive dysfunction and disturbance of consciousness
Khan et al., 2022
(Busan, Korea)
2022 MCI patients
n = 11 (0M, 11F);
Age: 61.58 ± 6.55y
Healthy people
n = 11 (0M, 11F);
Age: 55.92 ± 7.65y
Acupuncture therapy / 20-channels; 7.81 Hz Block design; working-memory task PFC Acupuncture therapy improved the hemodynamic responses of the patients. And the activated area of the MCI patients, as well as the connectivity, increased with acupuncture
Jeong et al., 2021
(Daejeon, Korea)
2021 MCI patients
n = 22;
Age: 50–75y
MCI patients
n = 22;
Age: 50–75y
Neurofeedback-acupuncture combined treatment Acupuncture treatment / Block design; delayed matching to sample task PFC /
Chen J. et al., 2020
(Hangzhou, China)
2020 PSCI patients
n = 28 (16M, 12F);
Age: 65.3 ± 6.8y
PSCI patients
n = 28 (17M, 11F);
Age: 64.5 ± 7.2y
Scalp acupuncture with cluster needling++drug treatment Drug treatment 12-channels; 10 Hz Resting-state design Frontal lobe, parietal lobe movement area Applying cluster needling of scalp acupuncture on top of drug treatment can significantly elevate the cerebral hemoglobin levels compared to patients treated with drug only
Sun, 2019
(Shenyang, China)
2019 PSCI patients
n = 30 (21M, 9F);
Age: 55.50 ± 10.38y
PSCI patients
n = 32 (22M, 10F);
Age: 58.34 ± 9.51y
Scalp acupuncture treatment+Routine rehabilitation training and drug treatment Routine rehabilitation training and drug treatment 16-channels; 10 Hz Task state design; resting-needling the scalp while retaining the needle-removing the needle PFC The statistics of changes in HbO2 concentration in the PFC under acupuncture state are higher than those under resting state
Ghafoor et al., 2019
(Busan, Korea)
2019 MCI patients
n = 12 (0M, 12F);
Age: 61.58 ± 6.55y
Healthy people
n = 12 (0M, 12F);
Age: 55.92 ± 7.65y
Acupuncture therapy / 20-channels; 7.81 Hz Block design; working memory task PFC The t-maps of MCI were enhanced. Furthermore, an increased FC in the PFC in MCI cases in comparison to before acupuncture was obtained, and an increasing trend in the graph theory parameters was observed
Eun-Sun et al., 2018
(Daejeon, Korea)
2018 MCI patients
n = 12;
Age: 40–80y
Healthy people
n = 12;
Age: 40–80y
Acupuncture treatment / 20-channels; 7.81 Hz Block design; working memory task PFC /
Motor dysfunction
Zhang et al., 2023
(Guangzhou, China)
2023 Patients with stroke hemiplegia
n = 20 (5M, 15F);
Age: 55.95 ± 16.98y
Patients with stroke hemiplegia
n = 20 (10M, 10F);
Age: 59.8 ± 11.63y
Auricular acupuncture; therapy+regular rehabilitation training; 5 times/week, 2 weeks Sham auricular acupuncture+regular rehabilitation training 5 times/week, 2 weeks 38-channels; 10Hz Resting-state design; 10 min M1 Auricular acupuncture has an ameliorative effect on upper limb motor deficits after stroke and that activation of the M1 region of the brain may be a key node in auricular acupuncture for treating upper limb dysfunction in stroke patients
Chen Y. F. et al., 2023
(Shanghai, China)
2023 Upper limb motor dysfunction after stroke
n = 10 (8M, 2F);
Age: 58.00 ± 10.31y
Healthy subjects
n = 8 (5M, 3F);
Age: 49.25 ± 6.82y
Tui Na: one-finger Zen manipulation at Hegu Tui Na: one-finger Zen manipulation at Hegu 21-channels; 19Hz Block design: six cycles: rest (20 sec); Tui Na (20 sec); rest (30 sec) PMC, M1, SMA, SAC Hemodynamics of contralateral SM1 was obviously enhanced, but there was no similar pattern in ipsilateral SM1; significant difference between lateralization index values for the affected arm and the less affected arm
Chen Y. F. et al., 2022
(Shanghai, China)
2022 Patients with stroke hemiplegia
n = 45
Patients with stroke hemiplegia
n = 45
rTMS + Tui Na + conventional rehabilitation rTMS + conventional rehabilitation 32-channels; 19Hz Resting-state: 8 min; Block design: six cycles: rest (10 sec); hand movements (15 sec); rest (20 sec) M1, PMC, SMA /
Tang et al., 2022
(Harbin, China)
2022 Patients with lower extremity motor dysfunction after stroke
n = 24, randomly divided into rehabilitation group
n = 12 (11M, 1F) (Age: 51.91 ± 4.63y) and acupuncture-rehabilitation group
n = 12 (11M, 1F) (Age: 53.50 ± 6.00y)
Healthy people
n = 10 (9M, 1F);
Age: 46.10 ± 11.00y
Rehabilitation group: routine rehabilitation training; acupuncture-rehabilitation group: acupuncture-rehabilitation intervention; 5 times/week, 4 weeks / 24-channels; 10Hz Walking task SMA, PMC, SMC Acupuncture with rehabilitation therapy can significantly improve the lower limb motor function and asymmetrical activation of SMC in stroke patients. The recovery of lower limb motor function may be related to the enhanced activation of affected PMC
Luo et al., 2022
(Mianyang, China)
2022 Observation group: patients with cerebral infarction
n = 43 (29M, 14F) (Age: 65.77 ± 5.36y) Motor imagination group: patients with cerebral infarction
n = 43 (30M, 13F) (Age: 66.42 ± 6.32y)
Patients with cerebral infarction
n = 43 (27M, 16F);
Age: 64.94 ± 6.86y
Observation group: basic medicines and routine rehabilitation+
exercise imaginary tai chi chuan steps Motor imagination group: basic medicines and routine rehabilitation+
exercise imaging therapy 5 times/week, 8 weeks
Basic medicines and routine rehabilitation 5 times/week, 8 weeks 22-channels Resting-state design SMA, PMC, SMC Tai chi chuan imagination therapy can effectively improve the movement, balance function and abnormal gait of patients’ lower limbs, and improve the ability of daily living of patients
Jang et al., 2020
(Daejeon, Korea)
2020 Parkinson’s patients
n = 13 (10M, 3F);
Age: 65.38 ± 7.81y
Parkinson’s patients
n = 13 (7M, 6F);
Age: 61.46 ± 8.33y
Acupuncture twice a week for 4 weeks+
conventional therapy
Conventional therapy 4 weeks 4.17Hz Task-state design S1, M1, SMA, PFC, PMC Acupuncture as an adjunct treatment can improve low-dose gait in PD patients and can improve balance, including activation of the cerebral cortex (PFC and auxiliary motor areas)
Mental and behavioral disorders
Zhang et al., 2021
(Shenzhen, China)
2021 Major depressive disorder patients
n = 47 (10M, 37F);
Age: 39.7 ± 12.24y
/ Acupuncture therapy / 52-channels; 10Hz Task state design; Verbal fluency task PFC A single session of acupuncture demonstrated a tendency to enhance the activation of the PFC in patients with severe depression
Wong et al., 2021
(Hong Kong, China)
2021 Depressed patients
n = 8;
Age: 44.8 ± 10.3y
Depressed patients
n = 12;
Age: 50.9 ± 11.1y
Acupuncture therapy+drug treatment Drug treatment 18-channels; 7.81Hz Resting-state design dPFC Depressed patients receiving acupuncture combined with antidepressants have the stronger rsFC in the dPFC compared to those using antidepressants alone
Xiang, 2017
(Chengdu, China)
2017 Generalized anxiety disorder patients
n = 12 (2M, 10F);
Age: 45.42 ± 13.41y
Healthy people
n = 12 (3M, 9F);
Age: 40.25 ± 11.99y
Acupuncture therapy / 250Hz Block design; Acupuncture stimulation-Acupuncture preparation-Acupuncture stimulation dPFC Acupuncture can activate the left dPFC in patients with generalized anxiety disorder, resulting in an immediate response characterized by an increase in HbO2 and a decrease in dHb
Miao, 2016
(Chengdu, China)
2016 Depressed patients
n = 8 (3M, 5F);
Age: 47.62 ± 6.72y
Depressed patients
n = 8 (2M, 6F);
Age: 48.12 ± 7.74y
Acupuncture therapy Drug treatment 4-channels; 250Hz Block design; emotional image processing task, N-back task dPFC After treatment, the HbO2 levels in the dPFC corresponding to three types of emotional images in both groups of patients increased. The HbO2 levels in the dPFC corresponding to sad images in the two groups of patients showed statistically significant differences at weeks 1, 2, 4, and 8
Chen, 2015
(Chengdu, China)
2015 Depressed patients
n = 8 (3M, 5F);
Age: 48.13 ± 9.75y
Depressed patients
n = 8 (2M, 6F);
Age: 47.9 ± 9.9y
Acupuncture therapy Drug treatment / Task state design; emotional image processing task dPFC The activation level of related brain areas in the first week of acupuncture was higher than that in the Western medicine group, while the activation intensity in the Western medicine group was higher than that in the acupuncture group in the second and eighth weeks
Insomnia
Xu Y. K. et al., 2022
(Beijing, China)
2022 Insomnia patients
n = 29 (10M, 19F) Age: 18–65y
Healthy people
n = 30 (10M, 18F) Age: 18–65y
Acupuncture therapy / 46-channels 6.25 Hz Emotional image processing task Frontal lobe, Parietal lobe The treatment of acupuncture for primary insomnia can regulate the concentration of HbO2 in the cerebral cortex by manipulating at specific acupoints, which can improve the function of loubus fromatis, orbitofrontal cortex and lobus parietalis related brain areas, enhance individual control of involuntary activation of extraneous stimuli, and regulate excessive arousal activity to improve insomnia
Wang et al., 2022
(Beijing, China)
2022 Young insomnia patients
n = 10 (Age: 18–35y) Middle aged insomnia patients
n = 12 (Age: 35–50y) Elderly insomnia patients
n = 8 (Age: 50–65y)
/ Acupuncture therapy 3 times/week, 2 weeks / 46-channels 6.25 Hz Event Experimental Design Frontal lobe The change of HbO2 in the frontal lobe after acupuncture supports the traditional Chinese medicine “Yin Self-half at forty years” and the theory of insomnia that “when Yang is high, the eyes are not closed, and that when Yin is high, the eyes are closed”
Pain disorders
Du et al., 2023a
(Shanghai, China)
2023 Patients with the cervical-shoulder syndrome
n = 20;
Age: 36.6 ± 7.2 y
/ The E-WAA was used to administer an electrical stimulation therapy that lasted for 5 min / 24-channels Block design PFC The FP and dPFC were linked to the analgesic modulation activated by the E-WAA
Du et al., 2023b
(Shanghai, China)
2023 Male patients with myofascial pain syndrome
n = 16;
Age: 47.19 ± 13.57
Male patients with myofascial pain syndrome
n = 15;
Age: 53.06 ± 17.16
Analgesic electrical stimulation combined with wrist-Ankle acupuncture / 24-channels 10 Hz Block design PFC Compared to no intervention, TENS based on wrist-ankle acupuncture can be effective in relieving pain in patients with MPS in terms of cerebral cortical hemodynamics
Shi et al., 2022
(Shanghai, China)
2022 Patients with trapezius myofascial pain syndrome
n = 25;
Age: 24–27y
Patients with trapezius myofascial pain syndrome
n = 25;
Age: 24–27y
Electronic wrist ankle needle stimulation for 5 min Electronic wrist ankle needle stimulation for 10 s / Block design PFC E-WAA have a great analgesic effect. The FP and dPFC were relative to the analgesia neuromodulation induced by the E-WAA
Pediatric diseases
Yang, 2023
(Chuzhou, China)
2023 Myopic children
n = 118 (2M, 10F);
Age: 6–14y
Healthy children
n = 128 (2M, 10F);
Age: 6–14y
Acupuncture therapy+
transcutaneous electrical acupoint stimulation therapy
/ / Task state design; visual task Visual cortex V1 V2 Compared to the normal vision group, the oxygenated hemoglobin in the V2 region of the myopic group during gaze movement and alternating gaze stages β Lower value
Wu, 2022
(Jinan, China)
2022 Anisometropic amblyopia children
n = 21 (13M, 8F);
Age: 6.52 ± 0.13y
Anisometropic amblyopia children
n = 24 (13M, 11F);
Age: 6.63 ± 0.13y
Transcutaneous electrical acupoint stimulation therapy Traditional acupuncture therapy 22-channels Event related design; visual stimulation task Visual cortex V1 V2 After traditional acupuncture or transcutaneous electrical acupoint stimulation intervention, HbO2-β value in the visual cortex regions of V1 and V2 were significantly higher than before the intervention
Zhuo et al., 2022
(Xi’an, China)
2022 Attention-deficit/hyperactivity disorder children
n = 39 (33M, 6F);
Age: 8.05 ± 1.187y
Attention-deficit/hyperactivity disorder children
n = 39 (31M, 8F);
Age: 8.56 ± 1.468y
Transcutaneous electrical acupoint stimulation therapy Sham transcutaneous electrical acupoint stimulation therapy 52-channels Block design; visual stimulation task Go/NO go task PFC After treatment, the HbO2 in PFC of the transcutaneous acupoint electrical stimulation group increased and was higher than that of the control group
Chai, 2020
(Jinan, China)
2020 Monocular anisometropic amblyopia children
n = 15 (7M, 8F);
Age: 7–12y
Healthy children
n = 38 (18M, 20F);
Age: 7–12y
Acupuncture therapy+ conventional therapy / 22-channels Block design; visual stimulation task PFC, OC When subjected to high spatial frequency checkerboard flipping stimulation, the β-value of the visual cortex increased compared to before acupuncture, and there was no statistically significant difference at baseline
Other disease
Zu et al., 2023
(Shanghai, China)
2023 Spinal cord injury patients
n = 15 (14M, 1F) Age: 33.98 ± 6.10
Healthy people
n = 25 (17M, 8F) Age: 25.33 ± 1.02
Aerobic exercise and wheelchair tai chi chuan Wheelchair tai chi chuan 63-channels 10 Hz Block design PFC, PMC, SMA, M1 The potential and advantages of wheelchair tai chi chuan in triggering cortical muscle coupling may optimize rehabilitation after spinal cord injury
Yu et al., 2023
(Shanghai, China)
2023 Patients with bilateral tinnitus
n = 18 (10M, 8F) Age: 27–67y
/ 20-min acupuncture session every other day for a total of ten times / 20-channels 10 Hz The auditory stimulus paradigm Temporal lobe Acupuncture increased the concentration of HbO2 in the temporal lobe of tinnitus patients, and affected the activation of the auditory cortex

M, male; F, female; FC, functional connectivity; PFC, prefrontal cortex; dPFC, dorsolateral prefrontal cortex; vPFC, ventrolateral prefrontal cortex; FPA, frontal area; FP, frontal polar; MC, motor cortex; OC, occipital cortex; SMC, sensorimotor cortex; BA, brodmann area; S1 ± primary somatosensory cortex; M1 ± primary motor cortex; S2 ± secondary somatosensory cortex.