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. 2024 Jan 22;53(3):27. doi: 10.3892/ijmm.2024.5351

Figure 3.

Figure 3

HOXA11-AS restores the effects of propofol on cell proliferation, apoptosis, migration and invasion in HCC. Hep3B and Huh-7 cells were untreated or treated with propofol, propofol + Vector or propofol + HOXA11-AS. (A) Expression of HOXA11-AS in Hep3B and Huh-7 cells was determined using reverse transcription-quantitative PCR. Proliferation of (B) Hep3B and (C) Huh-7 cells was evaluated using MTT assay. (D) Apoptosis of Hep3B and Huh-7 cells was analyzed via flow cytometry analysis. Protein expression levels of PCNA, cyclinD1 and C-Myc in (E) Hep3B and (F) Huh-7 cells were analyzed using western blotting. Protein expression levels of Bcl-2, Bax, c-caspase3 and c-PRRP in (G) Hep3B and (H) Huh-7 cells were analyzed using western blotting. (I) Migration and (J) invasion of Hep3B and Huh-7 cells were assessed using Transwell assay. Protein expression levels of E-cadherin, N-cadherin and Vimentin in (K) Hep3B and (L) Huh-7 cells were analyzed using western blotting. Experiments were repeated three times. *P<0.05. HOXA11-AS, HOMEOBOX A11 antisense RNA; c-, cleaved; PCNA, proliferating cell nuclear antigen; PARP, poly(ADP-ribose) polymerase 1.