TABLE 1.
Strains and plasmids used in this study
| Strain, plasmid, or cosmid | Characteristics | Reference and/ or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F− 80dlacZΔ(lacZYA-argF)U169 endA1 recA1 hsdR17 (rK− mK+) deoR thi-1 supE44 λ− gyrA96 relA1 | |
| Sφ874 | F−trp lacZ strA thi upp ΔsbcB-rfb | C. Whitfield (36) |
| AB1133 | K-12; thr-1 leuB6(Δgpt-proA)66 hisG4 argE3 thi-1 rfbD1 lacY ara-14 galK2 xyl-5 mtl-1 mgl-51 rpsL31 kdgK51 supE44 | P. Rick (42) |
| 21548 | AB1133; rfe::Tn10 Tcr | P. Rick (42) |
| LO6E6II | E. coli library clone of OG1RF | 57 |
| TX5159 | DH5α(pTEX5159) | Formerly called BO4B6I (57) |
| TX5169 | Sφ874(pTEX5159) | This study |
| E. faecalis | ||
| OG1RF | 35 | |
| TX52 | Clinical isolate from a patient with endocarditis | 57 |
| Plasmids and cosmids | ||
| pBeloBAC11 | Cosmid vector, F′ replicon, Cmr | H. Shizuya (57) |
| pBluescript SK (−) | High-copy-number cloning vector derived from pUC19, Apr | Stratagene |
| pTEX5159 | pBeloBAC11 with a 43-kb insert from OG1RF which contains a gene cluster for polysaccharide biosynthesis | 57 |
| pTEX5159A-U | pTEX5159::Tn7 Cmr Kmr | This study |
| pTEX5175 | 3,195-bp EcoRI-HindIII fragment of pTEX5159 subcloned in pBluescript SK (−), Apr | This study |