TABLE 5.
Characterization of transposon mutants
| Cosmid | Site of insertiona | Immuno- reactivityb
|
|
|---|---|---|---|
| DH5α | Sφ874 | ||
| pTEX5159G | orfde4 | − | |
| pTEX5159D | orfde4 | − | − |
| pTEX5159H | − | ||
| pTEX5159I | orfde5 | − | |
| pTEX5159E | orfde5 | − | DNGe |
| pTEX5159J | orfde5 | − | |
| pTEX5159K | orfde5 | − | |
| pTEX5159F | orfde5 | − | DNG |
| pTEX5159L | orfde4/5 | − | |
| pTEX5159M | − | ||
| pTEX5159A | orfde3 | + | + |
| pTEX5159B | orfde8 | + | − |
| pTEX5159C | orfde3-orfde4 | + | + |
| pTEX5159Nc | r7, 0.7 kb upstream of contig C | + | + |
| pTEX5159Oc | orfde2 | + | + |
| pTEX5159Qc | r7, 0.2 kb upstream of contig C | + | + |
| pTEX5159Pc | r7, 0.7 kb upstream of contig C | + | + |
| pTEX5159Rc | orfde2 | + | + |
| pTEX5159Sc | r6, 1.8 kb downstream of contig C | + | −?d |
| pTEX5159Tc | r6, 1.8 kb downstream of contig C | + | −d |
| pTEX5159Uc | r6, 1.9 kb downstream of contig C | + | −d |
a
ORF where the transposon had inserted. Sites of insertion of those that were not sequenced were estimated based on their PCR product sizes in relation to the ones that were sequenced.
b
Tested by using colonies of each clone in immunoblot analyses with patient serum.
c
Not been subjected to PCR amplification. The insertion site was determined by RED analysis.
d
The clone was transformed into Sφ874, resulted in large deletions in the insert, and was immunonegative.
e
Sφ874 transformants of pTEX5159E and pTEX5159F did not grow (DNG) when streaked from the primary transformation plates.