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. 2024 Jan 29;20(1):e1011145. doi: 10.1371/journal.pgen.1011145

Fig 1. Lp knockdown significantly impairs oogenesis and affects Vg localization.

Fig 1

(A) Following Lp knockdown, females develop a decreased number of eggs, as identified by dissection of ovaries of virgin females at 3–7 days PBM; each dot represents eggs per female; N = number of females, pooled from four biological replicates (Mann-Whitney: **** = p < 0.0001). (B) Triglycerides accumulate in the midgut and fat body of Lp-depleted females, and fail to accumulate in the ovaries; PBM = post blood meal; each dot represents tissues from three females; N = number of samples of three tissues, pooled from three biological replicates (REML variance component analysis by timepoint: **** = p < 0.0001; *** = p < 0.001). (C) Vg is persistently detectable in the fat body of Lp-depleted females and is decreased in the ovary at 48h PBM; samples of five tissues, representative blots (three biological replicates). (D) Transmission electron microscopy showing larger yolk granules (arrows pointing at darkly staining circles) in a dsLp ovarian follicle compared to dsLacZ at 24h PBM; scale bar = 2 μm (one biological replicate).