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. 2024 Jan 29;20(1):e1011145. doi: 10.1371/journal.pgen.1011145

Fig 3. Vg regulates Lp levels via TOR signaling.

Fig 3

(A) Vg expression relative to Rpl19 is reduced in the fat body 24h PBM upon 0.5 μL of 40 μM rapamycin treatment 2h PBM; Control is 2.4% DMSO in acetone; all values normalized to control; samples of ten tissues, four biological replicates (unpaired t test: **** = p < 0.0001). (B) TOR signaling is induced in the fat body upon Vg knockdown as shown by Western blotting of phospho-S6K levels; samples of five tissues, representative blot (three biological replicates). (C) The Vg-knockdown mediated upregulation of Lp at 24h PBM is suppressed in the fat body of dsVg females treated with 0.5 μL of 40 μM rapamycin 2h PBM; all values normalized to dsLacZ without rapamycin treatment; samples of ten tissues, four biological replicates (ANOVA). (D) Western blot showing phospho-S6K levels increase in the fat body at 24h PBM in Vg-depleted females, but this increase is repressed by rapamycin treatment; samples of five tissues, representative blot (four biological replicates). (E) Western blot showing fat body Lp levels are increased in Vg-knockdown females at 48h PBM, but this increase is repressed by rapamycin treatment; samples of five tissues, representative blot (four biological replicates).