TABLE 2.
Mobility of band 0.03 relative to those of other platelet GSL antigens
| GSL namea | No. of glycan residues | Mobility (Rf)b
|
GSL structure | |
|---|---|---|---|---|
| Solvent A | Solvent B | |||
| GlcCer | 1 | 0.59 | 0.85 | Glcβ1→1′Cer |
| LacCer | 2 | 0.40 | 0.75 | Galβ1→4Glcβ1→1′Cer |
| Gb3 | 3 | 0.25 | 0.65 | Galα1→4Galβ1→4Glcβ1→1′Cer |
| Gb4 | 4 | 0.16 | 0.55 | GalNAcβ1→3Galα1→4Galβ1→4Glcβ1→1′Cer |
| Lea-5 | 5 | 0.06 | 0.46 | Galβ1→3(Fucα1→4)GlcNAcβ1→3Galβ1→4Glcβ1→1′Cer |
| A-6 | 6 | 0.05 | 0.48 | GalNAcα1→3(Fucα1→2)Galβ1→3/4GlcNAcβ1→3Galβ1→4Glcβ1→1′Cer |
| Leb-6 | 7 | 0.04 | 0.40 | Fucα1→2Galβ1→3(Fucα1→4)GlcNAcβ1→3Galβ1→4Glcβ1→1′Cer |
| A-8 | 8 | 0.03 | 0.35 | GalNAcα1→3(Fucα1→2)Galβ1→(4GlcNAcβ1→3Galβ1→)24Glcβ1→1′Cer |
| Lea-7 | 7 | 0.01 | 0.24 | Galβ1→3(Fucα1→4)GlcNAcβ1→3Galβ1→4GlcNAcβ1→3Galβ1→4Glcβ1→1′Cer |
| Leb-8?c | 8 | NA | 0.14 | Fucα1→2Galβ1→3(Fucα1→4)GlcNAcβ1→3Galβ1→4GlcNAcβ1→3Galβ1→4Glcβ1→1′Cer |
| Lea-9?c | 9 | NA | 0.08 | Galβ1→3(Fucα1→4)GlcNAcβ1→3(Galβ1→4GlcNAcβ1→3)2Galβ1→4Glcβ1→1′Cer |
| Band 0.03 | 6–8 | 0.03 | 0.19 | Galα1→4Gal-(unknown oligosaccharide)-Galβ1→4Glcβ1→1′Cer |
a
By using the conventions of Henry et al. (44), the names of ABO and Le GSLs were abbreviated by listing the activity of the GSL (ABO or Le) followed by the size or total number of carbohydrate residues in the oligosaccharide moiety.
b
Rfs (mean ± 0.01) of GSL bands after separation in solvent A (C-M-water, 65:25:4 [vol/vol]) or solvent B (C–M–aqueous 0.2% CaCl2, 55:45:10 [vol/vol]). NA, not applicable.
c
The GSL bands hypothesized to reflect a possible Lea-8 and Leb-9 GSL structure were observed only after separation in solvent B.