TABLE 5.

Expression of Gb₃ and band 0.03 by individual platelet donors

Donor	Donor characteristics				% Platelet neutral GSLa			Ratioa,b		Stx-positive bands with Rf ofc:
	ABO/Rhd	P1d	Sexe	Age (yr)	CDH	Gb3	Gb4	Gb3/CDH	Gb3/Gb4	0.03	0.25
Individual donors
1	A+		F	27	19.2	41.5	31.3	2.15	1.32	+	+
2	A+	+	M	29	23.8	38.6	33.0	1.63	1.17		+
3	O−	+	M	36	67.6	8.7	9.2	0.10	0.94		−
4	A−	+	M	44	29.5	29.8	29.0	1.01	1.03	+	+
5	A+		F	21	55.3	14.6	20.1	0.26	0.73		+
6	A−		M	22	54.3	10.6	23.2	0.19	0.46		+
7	A+		F	39	25.9	32.6	26.4	1.26	1.23	+	+
8	A+	+	M	46	78.2	1.5	6.8	0.02	0.22		+
9	O+		M	28	44.6	17.2	27.1	0.39	0.63		+
10	O+		F	56	43.4	19.8	27.7	0.47	0.71		+
11	O+		F	47	48.7	14.5	26.0	0.30	0.55		+
12	A+		F	42	59.2	17.6	20.5	0.30	0.86		+
13	O−		F	23	25.5	28.8	34.0	1.14	0.85	+	+
14	O+	−	M	35	20.1	31.6	35.8	1.59	0.88		+
15	B−		F	22	37.4	24.4	29.3	0.62	0.83		+
16	B−	+	M	32	47.9	18.0	23.7	0.37	0.76		+
17	B+	+	M	40	36.4	21.4	22.6	0.59	0.95		+
18	AB−	−	F	30	21.8	35.6	33.4	1.62	1.06	+	+
19	A+	+	F	42	59.7	22.3	14.9	0.37	1.50		+
20	A+	+	M	39	60.0	15.5	8.1	0.26	1.91		+
21	O−	+	M	36	52.9	19.8	12.8	0.37	1.55		+
22	O+	+	M	25	25.8	5.8	10.3	0.23	0.56		+
23	O+	+	M	36	19.5	37.2	38.3	1.90	0.09		+
24	B−		F	19	33.9	27.4	28.7	0.81	0.95		+
25	B+	+	M	41	22.6	39.0	30.8	1.72	1.27		+
Groups of donorsf
All donors (n = 25)					40.5 ± 17.2	22.9 ± 11.0	24.1 ± 9.2
Band 0.03-positive donors (n = 5)					24.4 ± 4.0	33.7 ± 5.2	30.8 ± 3.1	1.44 ± 0.5	1.09 ± 0.2
Band 0.03-negative donors (n = 20)					44.6 ± 16.8	20.3 ± 11.0	22.4 ± 9.4	0.63 ± 0.6	0.91 ± 0.4
Pg					<0.02	<0.02	>0.05	<0.01	>0.10

^aScanning densitometry of DPA-stained HPTLC plates (n = 2). 

^bRatio of area (in square millimeters). 

^cSymbols: +, band identified; −, band not identified. 

^dDonor erythrocyte phenotype. 

^eF, female; M, male. 

^fValues shown for groups of donors are the means ± standard deviations. 

^gStudent t test. P values in blood that were also found to be statistically significant (P < 0.05) by the Mann-Whitney U-test (17) are shown in bold type.