TABLE 6.
Stx binding to platelets versus platelet Gb3 expression in individual platelet donors by flow cytometry and HPTLCa
| Sample | % Gb3b,c | Gb3/CDHb,d | % Stx-B positivee |
|---|---|---|---|
| 1 | 14.8 | 0.42 | 71.6 |
| 2 | 24.5 | 0.46 | 38.9 |
| 3 | 40.5 | 1.53 | 49.3 |
| 4 | 21.5 | 0.35 | 28.8 |
| 5 | 31.6 | 0.74 | 46.5 |
| 6f | ND | ND | 30.4 |
| 7 | 38.4 | 0.93 | 25.7 |
| 8f | ND | ND | 33.6 |
| 9f | ND | ND | 49.4 |
| 10 | 12.3 | 0.07 | 0.03 |
| 11f | ND | ND | 22.0 |
| 12 | 21.1 | 0.35 | 7.0 |
| 13f | ND | ND | 19.9 |
| 14 | 45.6 | 0.99 | 12.4 |
| 15 | 34.0 | 0.72 | 10.6 |
| 16 | 25.7 | 0.28 | 19.6 |
| 17 | 27.8 | 0.41 | 18.6 |
| 18 | 11.0 | 0.16 | 16.9 |
Percent Gb3 versus Stx-B positivity: r = +0.03 by Pearson product-moment correlation (17).
Scanning densitometry of DPA-stained HPTLC plates. ND, not done.
Percent Gb3 as percent total platelet neutral GSL.
Ratio of the area (in square millimeters) of Gb3 and CDH, as determined by scanning densitometry.
Percent platelets positive with FITC-labeled Stx-B subunits and PE-labeled MAb P2 (anti-CD41).
Inadequate sample for analysis.