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Tricine-SDS-PAGE of raw extract (1), active fraction (peak 4) from gel filtration column (2), purified peptide from C18 reverse phase-high-performance liquid chromatography (RP-HPLC) column (3), and size marker (4). A, Trypsinogen (23 kDa); B, lysozyme (14 kDa); and C, insulin (5.8 kDa).
