TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant features | Source or reference |
|---|---|---|
| E. coli K-12 | ||
| DH5α | High-efficiency transformation | BRLa |
| SM10 | Tra functions of IncP plasmids integrated into chromosome | 39 |
| B. pertussis | ||
| 18323 | Wild type | ATCC 9797 |
| TM1396 | 18323::bvgS(Con) | This study |
| TM1423 | 18323::bvgS(Con) ΔbvgR | This study |
| TM1424 | 18323::ΔbvgR | This study |
| Plasmids | ||
| pSS1615 | ptx-phoA Apr GmrrpsL oriT cos | 40 |
| pSS2000 | Apr Gmr rpsL oriT cos | 31 |
| pJM503 | bvgS(Con) Apr Gmr rpsL oriT cos | 32 |
| pTM012 | pUC19 with sacB BamHI cassette | This study |
| pTM023 | vrg6-phoA Apr KanrrpsL oriT cos | 31 |
| pTM025 | pSS2000 with the bvg SalI-XhoI fragment inserted into the SalI site | 31 |
| pTM119 | pTM025 with the 439-bp SalI fragment from bvg inserted into the SalI site | This study |
| pTM120 | pTM119 bearing an in-frame deletion of bvgR | This study |
| pTM126 | pTM120 bearing the sacB gene of B. subtilis | This study |
a
BRL, Bethesda Research Laboratories.