FIG. 1.

SDS-PAGE and Western blots of purified proteins. (A) Coomassie blue-stained gel of purified UspA2 heated for 10 min at 100°C (lane 2). (B) Coomassie blue-stained gel of purified UspA1 prepared either without heating of sample (lane 4) or with heating for 3 min at 100°C (lane 5), for 5 min at 100°C (lane 6), or heated for 10 min at 100°C (lane 7). (C) Western blot of purified UspA2 (lane 9) and purified UspA1 (lane 10) probed with the 17C7 MAb. Both proteins were heated for 10 min during sample preparation. Lanes: 1, 3, and 8, molecular size markers (in kilodaltons).