TABLE 1.
N-terminal sequences of peptides derived from cleavage of UspA2
UspA2 peptide sequencea | Matchb (Residue no.) | Cleavage agent |
---|---|---|
LLAEQQLNG | 92–100 | Trypsin |
ALESNVEEGL | 216–225 | Lys-C |
245–254 | ||
274–283 | ||
ALESNVEEGLLDLS | 274–288 | Trypsin |
505–515* | ||
AKASAANTDR | 378–387 | Chymotrypsin |
605–614* | ||
AATAADAITKNGN | 439–450 | Chymotrypsin |
SITDLGTKVDGFDGR | 458–472 | Lys-C |
VDALXTKVNALDXKVN | 473–488 | Trypsin |
AAQAALSGLFVPYSVGKFNATAALGGYGSK | 506–535 | CNBr |
Underlined residues denote mismatch with the nucleotide-derived amino acid sequence (2).
An asterisk indicates that the sequence also matches that sequence of UspA1. No asterisk indicates a match only with the nucleotide-derived amino acid sequence of UspA2.