Figure 6.
The effects of Aβ25-35 on hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), and lactate dehydrogenase (LDH) activities in young and old erythrocytes (RBCs). Cells were incubated at 25°C for 30 minutes in 10 mmol/L potassium phosphate buffer, pH 7.4, containing 0.9% NaCl, 5 mmol/L KCl, and 10 μmol/L Aβ25-35. Control was incubated with nontoxic Aβ35-25. Enzyme activities are expressed as IU/g hemoglobin (Hb); n = 8 to 18. Significant differences are indicated: *P < .05, **P < .01, and ***P < .001 as compared to young cells; P < .05 as compared to the old control (one-way analysis of variance [ANOVA] with Bonferroni’s multiple comparison test). Aβ indicates amyloid β.